MersV1, Main, Exploration, bibRecord, 002E23

Influence of chk1 and plk1 silencing on radiation- or cisplatin-induced cytotoxicity in human malignant cells

Identifieur interne : 002E23 ( Main/Exploration ); précédent : 002E22; suivant : 002E24

Influence of chk1 and plk1 silencing on radiation- or cisplatin-induced cytotoxicity in human malignant cells

Source :

Apoptosis [ 1360-8185 ] ; 2006-10-01.

RBID : ISTEX:285B94518A3F588C196E86E5ACDCF1C62B9610A1

English descriptors

KwdEn :
- Apoptosis, Cell cycle, Checkpoint kinase 1, Polo-like kinase 1.

Abstract

Abstract: The G2/M checkpoint is an attractive pathway for targeting and sensitizing tumor cells to cancer treatment. Abrogation of the G2/M checkpoint by targeting molecules, such as checkpoint kinase 1 (chk1), increases DNA breakage and sensitizes tumor cells to anti-tumoral agents. However, most of the previously described G2/M abrogators are actually targeting the G2-M border checkpoints rather than mitotic checkpoints. This prompted us to test the effects of combined targeting of chk1 and a critical regulator of mitosis, polo-like kinase 1 (plk1). Chk1 and plk1 were found to be co-expressed in 70% of primary neoplastic tissues we examined. Asynchronized tumor cells were treated with different DNA damaging-agents to activate G1/S, S or G2/M checkpoints. Either chk1 or plk1-specific antisense oligodeoxynucleotides (ASODN) enhanced DNA damaging agent-induced apoptosis. When used in combination, however, chk1- plus plk1-specific ASODN failed to produce synergistic effects. Moreover, selective targeting of plk1 or chk1 in tumor xenografts of mice by oncolytic adenovirus mutants demonstrated potent anti-tumoral efficacy in the presence of low dose cisplatin. Again, combined targeting of chk1 and plk1 did not further enhance anti-tumoral efficacy. We concluded that combined targeting of chk1 and plk1 was not superior to either targeting chk1 or plk1 alone, which suggested that chk1 and plk1 silencing might overlap in their mechanism of action. Whether combined targeting of chk1 with other, more specific mitotic regulators would synergistically sensitize tumor to anti-neoplastic therapeutics needs to be further clarified.

Url:

https://api.istex.fr/ark:/67375/VQC-1FVMFMT5-F/fulltext.pdf

DOI: 10.1007/s10495-006-9421-4

Affiliations:

République populaire de Chine

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to stream Main, to step Curation: 002E23

Le document en format XML

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<author><name sortKey="Lu, Yunping" sort="Lu, Yunping" uniqKey="Lu Y" first="Yunping" last="Lu">Yunping Lu</name>
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<author><name sortKey="Zhuang, Liang" sort="Zhuang, Liang" uniqKey="Zhuang L" first="Liang" last="Zhuang">Liang Zhuang</name>
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<author><name sortKey="Wang, Shixuan" sort="Wang, Shixuan" uniqKey="Wang S" first="Shixuan" last="Wang">Shixuan Wang</name>
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<author><name sortKey="Zhou, Jianfeng" sort="Zhou, Jianfeng" uniqKey="Zhou J" first="Jianfeng" last="Zhou">Jianfeng Zhou</name>
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<front><div type="abstract" xml:lang="en">Abstract: The G2/M checkpoint is an attractive pathway for targeting and sensitizing tumor cells to cancer treatment. Abrogation of the G2/M checkpoint by targeting molecules, such as checkpoint kinase 1 (chk1), increases DNA breakage and sensitizes tumor cells to anti-tumoral agents. However, most of the previously described G2/M abrogators are actually targeting the G2-M border checkpoints rather than mitotic checkpoints. This prompted us to test the effects of combined targeting of chk1 and a critical regulator of mitosis, polo-like kinase 1 (plk1). Chk1 and plk1 were found to be co-expressed in 70% of primary neoplastic tissues we examined. Asynchronized tumor cells were treated with different DNA damaging-agents to activate G1/S, S or G2/M checkpoints. Either chk1 or plk1-specific antisense oligodeoxynucleotides (ASODN) enhanced DNA damaging agent-induced apoptosis. When used in combination, however, chk1- plus plk1-specific ASODN failed to produce synergistic effects. Moreover, selective targeting of plk1 or chk1 in tumor xenografts of mice by oncolytic adenovirus mutants demonstrated potent anti-tumoral efficacy in the presence of low dose cisplatin. Again, combined targeting of chk1 and plk1 did not further enhance anti-tumoral efficacy. We concluded that combined targeting of chk1 and plk1 was not superior to either targeting chk1 or plk1 alone, which suggested that chk1 and plk1 silencing might overlap in their mechanism of action. Whether combined targeting of chk1 with other, more specific mitotic regulators would synergistically sensitize tumor to anti-neoplastic therapeutics needs to be further clarified.</div>
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Influence of chk1 and plk1 silencing on radiation- or cisplatin-induced cytotoxicity in human malignant cells

Influence of chk1 and plk1 silencing on radiation- or cisplatin-induced cytotoxicity in human malignant cells

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri